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It should be pointed out that Griffith [11] originally validated this method in plasma where the artifactual oxidation of GSH is minimal, i.e., tolerable, because the GSH/GSSG ratio in plasma is much lower than in cells (~2 instead of ~400) [3], but this procedure has later been applied broadly to whole blood, red cells or other tissues in which GSH massively exceeds GSSG without proper modification

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They demonstrated an increase in GSH content, due to the augmented transcription of GCL