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The sourced reference for subcutaneous injection is CDC's Pink Book chapter on vaccine administration (last reviewed April 8, 2024): a 5/8-inch needle, 23 to 25 gauge, inserted at a 45-degree angle into the fatty tissue over the triceps (or the thigh), with the site cleaned with alcohol and allowed to dry

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Development of an optimized refolding process for recombinant Ala-Glu-IGF-1 [PMID: 1287661] Laboratory literature summary: Development of an optimized refolding process for recombinant Ala-Glu-IGF-1