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Using co-immunoprecipitation (CO-IP), the study confirmed that FOXO4-DRI prevents FOXO4 binding to p53, facilitating phosphorylated p53 nuclear exclusion, which triggers BAX upregulation and cleaved caspase-3 activation — driving selective apoptosis of senescent endothelial cells

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Outcomes included improved load to failure, motor function indices, muscle myofibril and macroscopic diameters, and reduced atrophy and subcutaneous gapping [33,36,48]