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Single color imaging was performed using a previously described imaging buffer 57 , 100 mM cysteamine (Sigma-Aldrich, #30070), 5% glucose (Sigma-Aldrich, #G8270), 1% Glox (0.5 mg/mL glucose oxidase, 40 mg/mL catalase (Sigma-Aldrich, #G2133 and #C100)) in PBS