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In addition, Western blot analysis revealed enhanced activation of the NRF2–SOD2 antioxidant axis, suppression of NF-κB signaling (p-p65), decreased expression of the muscle atrophy markers MuRF1 and FBXO32, and maintenance of MHC protein levels (Fig

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To assess if the metabolism of TNBS by NAPDH-linked enzymes in aerobic solution results in formation of ROS, we determined the formation of hydrogen peroxide in an aqueous solution (PBS) containing 0.25 mm NADPH, TNBS (1 mm) and GSSG reductase (15 μU/ml) over time